亚洲欧美精品伊人久久,亚洲成人一区在线,东北老熟女被爆操贵在真实,久久久久国产精品人妻aⅴ四季

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>CRL-27842V6.11 JHU-67 人胚腎細(xì)胞

2V6.11 JHU-67 人胚腎細(xì)胞

簡要描述:CRL-2784 2V6.11 JHU-67 人胚腎細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞,。細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

  • 產(chǎn)品型號:CRL-2784
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2025-08-14
  • 訪  問  量:1978

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2784 2V6.11 JHU-67 人胚腎細(xì)胞

ATCC® Number:  CRL-2784™

Designations:  2V6.11 [JHU-67]

Depositors:   G Ketner

Biosafety Level: 2 [Cells containing Adenovirus viral DNA sequences ]

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

CRL-2784 2V6.11 JHU-67 人胚腎細(xì)胞

Source: Organ: kidney

Cell Type: epithelialtransformed with adenovirus 5 DNA

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Restrictions: Part of the Johns Hopkins Special Collection

Isolation:  Isolation date: 2001

Age:  fetus

Comments: This line was derived from the human embryonic kidney line, 293 in 2001. 293 cells were transfected with the plasmid pVgRXR bearing a Zeocin-resistance selectable marker to obtain 293VgRXR cells. This population was subsequently transfected with the plasmid pEKORF6 containing Ad5 E4 ORF 6 that encodes E4 34K. pEKORF6 is a derivative of the plasmid pIndHydro that contains a hygromycin resistance gene. The vectors contain SV40 viral DNA sequences. The 2V6.11 cell line was originally selected in hygromycin-containing medium and cloned by single-colony isolation with a cloning cylinder. The 2V6.11 cells inducibly express the human adenovirus E4, 34kDa protein (E4 ORF6). 2V6.11 cells exhibit little or no constitutive E4 biologic activity. Induction by ponasterone A, an ecdysone analog, however, results in E4 biologic activity and levels of E4 34kDa protein that are detectable by immunoblotting. These cells can be used as a tool to study the biology of the adenoviral E4 oncoprotein.

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Growth Conditions: The depositor recommends periodic hygromycin selection.

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

An inoculum of 5 X 10(3) to 1 X 10(4) viable cells/cm2 is recommended.

Incubate cultures at 37°C.

Interval: Maintain cultures at a cell concentration between 2 X 10(4) and 2 X 10(5) cells/cm2.

Subc*tion Ratio: A subc*tion ratio of 1:6 to 1:10 is recommended

Medium Renewal: Two to three times weekly

Preservation:  Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time:  23 hrs.

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

source culture:ATCC JHU-67

Related Links

NCBI Entrez Search

Make a Deposit

Frequently Asked Questions

Material Transfer Agreement

Technical Support

Related Cell Culture Products


CRL-2784



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
久久国产精品99毛片| 日韩少妇精品不卡| 精品一卡2卡三卡4卡乱码精品视频| 一本一道久久综合狠狠老| 久久亚洲AV成人无码国产电影| 精品久久人人妻人人做精品| 精品人妻码一区二区三区| 国产精品高清视亚洲中文| 18禁黄网站禁片免费观看不卡| 疯狂做受xxxx高潮欧美日本| 国产末成年女AV片一区二区| 一区二区三区四区三级片视频在线观看| 免费在线观看黄色网址| 大香蕉搞搞网| 天堂资源中文最新版在线一区| 欧美黑人巨大videos精品| 你懂的视频在线播放| 永久域名99精品| Av2015| 色狠狠老熟女| 国产亚洲欧美日韩久久| 在线观看免费毛片视频| 成人免费精品网站在线观看影片| 嗯嗯热亚洲69| 久久丫精品国产亚洲av| 激情综合五月开心婷婷| 蜜芽国产欧美一区二区三区| 艳z门照片无码av| 黑人大荫蒂老太大| 色综合久久一区二区三区| 亚洲日韩精品无码专区加勒比| 五月丁香花开婷婷| 国偷自产一区二区免费视频| 久久亚洲中文字幕精品一区| 中文最新版地址在线| 日本人妻BBwBBwBBW| 精品无码AV无码免费专区| 性爱视频日本免费在线播放网址| 久久人妻av一区二区软件| 亚洲加勒比少妇无码AV| 无码国产偷倩在线播放老年人|