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簡要描述：CRL-2553 Panc 02.03 人胰腺癌細胞，原代細胞|細胞系|細胞株|菌種；細胞庫管理規(guī)范，提供的細胞株背景清楚，提供參考文獻和培養(yǎng)條件！
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CRL-2553 Panc 02.03 人胰腺癌細胞
ATCC® Number: CRL-2553™ Price: $417.00
Designations: Panc 02.03
Depositors: EM Jaffee
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens （human）
Morphology: epithelial
Source: Organ: pancreas
Disease: adenocarcinoma
Cellular Products: cytokeratins 7 and 18 [50655]
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-2553 Panc 02.03 人胰腺癌細胞
Tumorigenic: Yes
Oncogene: K-ras +
Antigen Expression: MHC class I +; MHC class II - [50655]
DNA Profile （STR）: Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 11
D5S818: 12,13
D7S820: 9,10
THO1: 6
TPOX: 9,12
vWA: 17
Age: 70 years
Gender: female
Ethnicity: White
Comments: Panc 02.03 is a pancreatic adenocarcinoma epithelial cell line derived in 1995 from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma.
The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. [50655]
The cells have a reported plating efficiency of 100%. [50655]
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%
Atmosphere: air, 95%; carbon dioxide （CO2）, 5%
Temperature: 37.0°C
Subculturing: Protocol:
1.Remove and discard culture medium.
2.Briefly rinse the cell layer with 0.25% （w/v） Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）.
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5.Add appropriate aliquots of the cell suspension to new culture vessels.
6.Incubate cultures at 37°C.
Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% （v/v） DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 28.2 hrs [50655]
Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2001
recommended serum:ATCC 30-2020
References: 50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602
CRL-2553
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