亚洲欧美精品伊人久久,亚洲成人一区在线,东北老熟女被爆操贵在真实,久久久久国产精品人妻aⅴ四季

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>小鼠腫瘤細(xì)胞、癌細(xì)胞>TIB-67J774A.1 小鼠腹水單核細(xì)胞瘤

J774A.1 小鼠腹水單核細(xì)胞瘤

簡要描述:TIB-67 J774A.1 小鼠腹水單核細(xì)胞瘤,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

  • 產(chǎn)品型號:TIB-67
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2025-08-14
  • 訪  問  量:2657

產(chǎn)品分類

Product Category

詳細(xì)介紹

TIB-67 J774A.1 小鼠腹水單核細(xì)胞瘤,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)i優(yōu)培養(yǎng)條件

TIB-67 J774A.1 小鼠腹水單核細(xì)胞瘤

ATCC® Number:TIB-67™  Price:$256.00
Designations:J774A.1

Depositors:P Ralph

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:macrophage
J774A.1 小鼠腹水單核細(xì)胞瘤


Source:Tissue: ascites
Strain: BALB/cN
Disease: reticulum cell sarcoma
Cell Type: monocyte/macrophage macrophage;


Cellular Products:interleukin 1 beta

lysozyme [1080]



Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
TIB-67

Isolation:La Jolla California, United States
Isolation date: 1968


Applications:Biological response [92560]

transfection host (Roche FuGENE® Transfection Reagents)



Receptors:complement (C3), expressed [1135]

Fc receptor, IgG, high affinity I (Fcgr1), expressed [13710]



Age:adult

Gender:female

Comments:J774A.1 cells are active in antibody dependent phagocytosis [Pubmed: 1101071]. Their growth is inhibited by dextran sulfate, PPD and LPS [Pubmed: 318922]. They synthesize large amounts of lysozyme and exhibits minor cytolysis but predominantly antibody-dependent phagocytosis. Interleukin 1 beta (Il1b) is synthesized continuously by this line.

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing:Protocol: Subcultures are prepared by scraping.

For a 75 cm2 flask, remove all but 10 ml of the culture medium. (adjust volume accordingly for different culture vessels) Dislodge cells from the flask substrate with a cell scraper, aspirate and dispense into new flasks.


Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended
Medium Renewal: Replace or add medium two or three times weekly


Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Doubling Time:17 hours

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

purified RNA:ATCC TIB-67R



References:1080: Ralph P, et al. Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines. J. Exp. Med. 143: 1528-1533, 1976. PubMed: 1083890

1135: Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031

1136: Ralph P, Nakoinz I. Direct toxic effects of immunopotentiators on monocytic myelomonocytic, and histiocytic or macrophage tumor cells in culture. Cancer Res. 37: 546-550, 1977. PubMed: 318922

13710: Sears DW, et al. Molecular cloning and expression of the mouse high affinity Fc receptor for IgG1. J. Immunol. 144: 371-378, 1990. PubMed: 2136886

22827: Ralph P, et al. Reticulum cell sarcoma: an effector cell in antibody-dependent cell- mediated immunity. J. Immunol. 114: 898-905, 1975. PubMed: 1089721

22896: Ralph P, Nakoinz I. Phagocytosis and cytolysis by a macrophage tumour and its cloned cell line. Nature 257: 393-394, 1975. PubMed: 1101071

32697: Knowlton KU, et al. A mutation in the puff region of VP2 attenuates the myocarditic phenotype of an infectious cDNA of the woodruff variant of coxsackievirus B3. J. Virol. 70: 7811-7818, 1996. PubMed: 8892902

32883: Schissel SL, et al. Zn2+-stimulated sphingomyelinase is secreted by many cell types and is a product of the acid sphingomyelinase gene. J. Biol. Chem. 271: 18431-18436, 1996. PubMed: 8702487

92560: Standard Practice for Testing for Biological Responses to Particles in Vitro. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 1903-98R03.

















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国产精品尤物在线| 麻花传媒免费网站在线观看| 人人爽片AV| 人人妻熟妇中年乱子伦A| 粗大挺进朋友人妻淑娟| 精品毛卡卡1卡2卡3麻豆| 色窝窝亚洲av网在线观看| 91精品人人妻人人做人人爱| 毛片无限精品天堂粗大无码| 蜜桃网站入口在线进入| 人人爽人人添人人澡| 久久无码无码久久综合综合| 亚洲av成人一区二区三区av| 亚洲精品无码不卡在线播放he| 久久久www成人免费精品| 国产内射999视频一区| 国产Av熟女大屁股www屁股| 免费电影理论片一区二区三区| 久操五月视频| 久久水蜜桃网国产免费网2区| 久久精品麻豆日日躁夜夜躁| 四虎成人精品在永久在线| 亚洲精品无码久久不卡| 国内少妇偷人精品免费| 老熟妇仓乱一区二区日本有码| 午夜裸体性播放| 久激情内射婷内射蜜桃| 野花视频在线观看免费观看最新| 中国女人和老外的毛片| 无码一区二区三区免费视频| 最近的中文字幕在线看视频| 婷婷 色五月 亚洲 色图| 亚洲一卡二卡无码| 午夜精品久久99蜜桃的功能介绍| 欧美精产国品一二三区别| 亚洲老骚肥逼| 衣服被扒开强摸双乳18禁网站| 国产av综合影院| 黑人又大又硬让人受不了| 国产午夜精品一区二区三区不卡| 中文亚洲一区二区三区有码|